滑液支原体WVU1853株免疫相关膜蛋白的初步分析

doi:10.11843/j.issn.0366-6964.2017.02.015

Abstract

Abstract:

As a major component of membrane structure, membrane proteins not only have important physiological functions in the life of pathogen, but some of them are closely related to pathogen infection and immune response. Therefore, the main aim of this study was to isolate, screen and identify unknown possible immunogenicity proteins of Mycoplasma synoviae (MS) using immunoproteomics approach, which will lay a foundation not only for the study of infection and immune mechanism of MS but also for the establishment of diagnosis and prevention methods of related diseases. Chickens and New Zealand rabbits were immunized with MS cultured to mid-to-late logarithmic growth phase, respectively. Then the antiserum against MS from the chicken and the rabbit were prepared. Based on these, the membrane proteins of MS were isolated and then separated by two-dimensional electrophoresis. Finally the immunogenicity membrane proteins were screened and identified by Western blot combined with mass spectrum. The results showed that among the eight screened protein spots, three protein spots were identified as elongation factor EF-Tu, the rest of protein spots were identified as pyruvate decarboxylase alpha subunit, beta subunit, NADH oxidase, histidyl-tRNA synthetase and dihydrolipoyl dehydrogenase, respectively. Among them, it was confirmed for the first time that the pyruvate decarboxylase alpha subunits and beta subunits were the immune-related membrane proteins of MS, whereas the histidyl-tRNA synthetase need to be confirmed by further studies because protein score and protein Score CI% were too low. Several immune-related membrane proteins of MS strain WUV1853 were identified, which will be useful for further study on the biological function of target protein and development of new vaccines and diagnostic reagents.

CLC Number:

S852.62

BAO Shi-jun, DING Xiao-qin, XING Xiao-yong, FU Xiao-ping, XUE Hui-wen, WEN Feng-qin. Preliminarily Analysis of Immune-related Membrane Proteins from Mycoplasma synoviae WVU1853 Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 316-323.

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Preliminarily Analysis of Immune-related Membrane Proteins from Mycoplasma synoviae WVU1853 Strain

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